ATP5D

Alternative Names: ATP5D, ATP synthase subunit delta mitochondrial, F-ATPase delta subunit

F-ATPase delta, also known as ATP5D, is a crucial enzyme in the process of ATP synthesis within mitochondria. It utilizes a proton gradient across the inner membrane to generate ATP. The enzyme is part of ATP synthase, which is made up of two main complexes: F1, the catalytic core, and Fo, the proton channel. The F1 complex consists of five subunits, while the Fo complex is composed of three main subunits. ATP5D specifically is the delta subunit of the catalytic core.

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  • Sku: PSB-enz-143-1µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human ATP Synthase Subunit D, Mitochondrial is a protein component of the ATP synthase enzyme complex located in the mitochondria. Belonging to the ATP synthase family, this subunit plays a key role in the synthesis of adenosine triphosphate (ATP) through the process of oxidative phosphorylation. With a molecular weight of approximately 16 kDa, Recombinant Human ATP Synthase Subunit D is involved in the generation of cellular energy and is crucial for proper mitochondrial function.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
17.3 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ATP5D protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA AHVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ATP5D

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