ATG5

Alternative Names: Autophagy protein 5, APG5, APG5-like, APG5L, hAPG5, Apoptosis-specific protein, ATG5, ASP.

ATG5 is a crucial member of the ATG gene family involved in autophagy, which is the breakdown of cytoplasmic contents in the lysosome. This protein is essential for autophagy as it combines with ATG12 and contributes to the formation of autophagosomes on the isolation membrane. ATG5 also plays a role in apoptosis, appearing later in the process, after caspase activity.

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  • Sku: PSB-pro-454-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Autophagy Related 5 (ATG5) is a protein that plays a vital role in the process of autophagy, a cellular mechanism responsible for recycling and degrading unnecessary or damaged cellular components. Belonging to the ATG protein family, ATG5 functions as a key regulator in the formation of autophagosomes, which are double-membrane vesicles involved in capturing and delivering cargo to the lysosomes for degradation. With a molecular weight of approximately X kilodaltons, ATG5 acts as a critical component in maintaining cellular homeostasis and is involved in various physiological and pathological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
34.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ATG5 at a concentration of 0.25mg/ml containing 20mM Tris-HCl, pH 8.0, 1mM DTT , 0.2M NaCl and 40% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLPRVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNITVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLKEVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ATG5

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