ATF1

Alternative Names: Activating transcription factor 1, cyclic AMP-dependent transcription factor ATF-1, FUS/ATF-1, Protein TREB36, EWS-ATF1.

ATF1 is a transcription factor that relies on cyclic-AMP for its activity and is found in various cell types. It can form dimers with CREB. MSK1 and MSK2 protein kinases play a crucial role in the stress-induced phosphorylation of CREB and ATF1 in early embryonic fibroblasts. The activation of c-jun expression by epidermal growth factor requires the presence of ATF1 and MEF2 binding sites in the c-jun promoter.

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  • Sku: PSB-pka-019-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Activating Transcription Factor-1 (ATF-1) is a protein that plays a crucial role in regulating gene expression. Belonging to the ATF/cAMP response element-binding (CREB) protein family, ATF-1 functions as a transcription factor by binding to specific DNA sequences known as cAMP response elements (CREs). With a molecular weight of approximately [insert weight], ATF-1 modulates the transcriptional activity of various genes involved in diverse cellular processes. Its significance lies in its ability to regulate gene expression, making it a valuable tool in studying cellular pathways and understanding the molecular mechanisms underlying various physiological and pathological conditions.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
31.8 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ATF1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 5mM DTT, 2mM EDTA and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSD SIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMS VPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQ VVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKE YVKCLENRVA VLENQNKTLI EELKTLKDLY SNKSV

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ATF1

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