ASF1B

Alternative Names: Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b, CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B.

ASF1B is a histone chaperone protein that plays a role in regulating the structure of chromatin. It is similar to a protein found in yeast called anti-silencing function-1. ASF1B is phosphorylated by cell cycle-regulated kinases from the tousled-like kinase family, and this phosphorylation is important for its function. By ensuring a steady supply of histones at sites of nucleosome assembly, ASF1B helps to modulate the nucleosome structure of chromatin. Additionally, ASF1B works together with a protein called CAF-1 to promote the assembly of chromatin during DNA replication. ASF1B is mainly found in high levels in the testis, and in lower levels in the colon, small intestine, and thymus. It is essential for spermatogenesis.

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  • Sku: PSB-pro-1163-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human ASF1 Anti-Silencing Function 1 Homolog B is a protein belonging to the ASF1 family, which plays a significant role in chromatin assembly and histone chaperoning. With a weight of approximately X kDa, this protein is involved in the regulation of gene expression and maintenance of genome stability. Its intricate involvement in these cellular processes makes it a subject of scientific interest for understanding chromatin dynamics and epigenetic regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 23.4 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation ASF1B protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTPIKGLGLPGCI PGLLPENSMD CILEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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ASF1B
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