ARHGDIA

Alternative Names: Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1, RHOGDI, RHOGDI-1, MGC117248.

ARHGDIA is a member of the RAS gene superfamily and plays a role in inhibiting the dissociation of GDP from Rho proteins, thereby preventing their activation. It is located in the cytoplasm. Phosphorylation of ARHGDIA at Ser 101 by p21-activated kinase can inhibit its activity and potentially regulate multiple ARHGDIA target proteins through positive feedback.

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  • Sku: PSB-pro-002-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Rho GDP Dissociation Inhibitor (GDI) Alpha is a protein that belongs to the Rho GDI family. With a molecular weight of approximately X kDa, this protein plays a significant role in regulating the activity of Rho GTPases. Rho GDI Alpha functions by inhibiting the dissociation of GDP (guanosine diphosphate) from Rho GTPases, thereby maintaining them in an inactive state. This protein's role in the regulation of cellular processes makes it a subject of interest in various scientific studies and applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
22.9 kDa
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ARHGDIA solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSVNYKPPAQ KSIQEIQELD KDDESLRKYK EALLGRVAVS ADPNVPNVVV TGLTLVCSSA PGPLELDLTG DLESFKKQSF VLKEGVEYRI KISFRVNREI VSGMKYIQHT YRKGVKIDKT DYMVGSYGPR AEEYEFLTPV EEAPKGMLAR GSYSIKSRFT DDDKTDHLSW EWNLTIKKDW KD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ARHGDIA

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