ARG1, Active

Alternative Names: Arginase-1, Arginase-1 liver, Arginase-1 liver A I, Al, ARG 1, ARG1, Arginase 1, Arginase liver, Arginase type I, Arginase1, Liver, Liver type arginase, Type I arginase.

Arginase-1 is an enzyme involved in the urea cycle, breaking down arginine into ornithine and urea. There are two forms of mammalian arginase with varying characteristics. Arginase-1 is mainly found in the liver and deficiency of this enzyme can result in a condition called argininemia, characterized by elevated ammonia levels.

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  • Sku: PSB-enz-1120-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Arginase-1, Active is a member of the arginase family of enzymes. It is a protein with a molecular weight of approximately 35 kDa. This enzyme plays a key role in the urea cycle, catalyzing the hydrolysis of arginine to ornithine and urea. Recombinant Human Arginase-1, Active is commonly used in research and biotechnological applications due to its ability to regulate arginine metabolism.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0 % by SDS-PAGE
Activity
 Specific activity is > 150,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation ARG1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT and 100mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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ARG1, Active
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