ARF5

Alternative Names: ARF5, ADP-ribosylation factor 5.

ARF5 is a small guanine nucleotide-binding protein that increases cholera toxin activity. It regulates spectrin-golgi membrane interactions and links actin to the golgi through rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is also involved in vesicular transport and functions by activating phospholipase D.

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  • Sku: PSB-pro-245-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human ADP-Ribosylation Factor 5 (ARF5) is a small GTP-binding protein that belongs to the ADP-ribosylation factor family. With a molecular weight of approximately 21 kDa, ARF5 plays a significant role in regulating intracellular vesicular trafficking and membrane dynamics. It functions as a molecular switch, cycling between an inactive GDP-bound state and an active GTP-bound state, thereby controlling various cellular processes such as membrane trafficking, cytoskeletal organization, and signal transduction pathways. ARF5's involvement in these fundamental cellular mechanisms makes it a valuable target for studying intracellular transport and its potential implications in diseases.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
22.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ARF5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ARF5

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