ARF4

Alternative Names: ARF2, ARF4, ADP-ribosylation factor 4

ARF4 is a member of the ARF gene family, which encodes small guanine nucleotide-binding proteins known for stimulating the ADP-ribosyltransferase activity of cholera toxin. These proteins also play a role in vesicular trafficking and as activators of phospholipase D. The ARF gene family includes five ARF proteins and eleven ARF-like proteins, making it a part of the RAS superfamily. This superfamily is further classified into class I, class II, and class III, with ARF4 belonging to class II. Each class shares a common gene organization. The ARF4 gene spans about 12kb and has 6 exons and 5 introns. Among the human ARFs, the ARF4 gene is the most distinct.

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  • Sku: PSB-pro-066-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human ADP-Ribosylation Factor 4 (ARF4) is a protein belonging to the ADP-ribosylation factor (ARF) family. With a molecular weight of approximately 20 kDa, ARF4 plays a significant role in intracellular vesicular trafficking and regulation of membrane dynamics. It functions as a GTP-binding protein, cycling between an inactive GDP-bound state and an active GTP-bound state. ARF4 is involved in various cellular processes, including protein sorting, membrane remodeling, and organelle biogenesis. Its precise functions and interactions make it a valuable target for studying cellular trafficking pathways and potential therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The ARF4 solution (0.5 mg/ml) in 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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ARF4
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