ARF3

Alternative Names: ARF3, ADP-ribosylation factor 3.

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These small guanine nucleotide-binding proteins play a role in stimulating the ADP-ribosyltransferase activity of cholera toxin and are involved in vesicular trafficking and activation of phospholipase D. ARF3 is an allosteric activator of the catalytic subunit of cholera toxin, functioning as an ADP-ribosyltransferase. It also participates in protein trafficking by modulating vesicle budding and uncoating in the Golgi apparatus. The ARF3 gene consists of 5 exons and 4 introns.

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  • Sku: PSB-pro-933-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human ADP-Ribosylation Factor 3 (ARF3) is a small GTPase protein that belongs to the ADP-ribosylation factor family. With a molecular weight of approximately 21 kDa, ARF3 plays a crucial role in intracellular vesicular trafficking and membrane dynamics. It regulates the formation, budding, and fusion of vesicles involved in various cellular processes, including protein trafficking, organelle biogenesis, and signal transduction. ARF3 acts as a molecular switch, cycling between an inactive GDP-bound state and an active GTP-bound state, thereby controlling the recruitment and activation of effector proteins involved in vesicle trafficking pathways. Understanding the functional mechanisms of ARF3 can provide valuable insights into cellular processes and potentially aid in the development of therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
22.8 kDa
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ARF3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ARF3

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