ARF1

Alternative Names: ADP-ribosylation factor 1, ARF-1.

ARF1 is a guanine nucleotide-binding protein involved in facilitating cholera toxin enzymatic activities. It is essential and widespread in eukaryotes, playing a role in vesicular transport and functioning through activating phospholipase D. Additionally, ARF1 is crucial for maintaining membrane traffic, organelle integrity, and establishing reversible associations with membranes. ARF1's interactions with membrane phospholipids are also distinct and significant.

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  • Sku: PSB-pro-867-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human ADP-Ribosylation Factor 1 (ARF1) is a small GTP-binding protein that plays a critical role in regulating intracellular vesicular trafficking and membrane dynamics. Belonging to the ARF family, ARF1 acts as a molecular switch, cycling between an inactive GDP-bound form and an active GTP-bound form, thereby controlling various cellular processes. With a molecular weight of approximately 20 kDa, ARF1 functions by recruiting specific effector proteins to specific subcellular compartments, facilitating the formation of transport vesicles and maintaining cellular homeostasis.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ARF1 protein solution (1mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ARF1

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