APOA1, His

Alternative Names: Apo-AI, ApoA-I, APOA1, Apolipoprotein A-I, MGC117399.

APOA1 is a human protein crucial in lipid metabolism as the primary component of HDL. It facilitates the removal of cholesterol from tissues to be excreted by the liver and aids in the formation of plasma cholesteryl esters as a cofactor for LCAT. Additionally, APOA1 plays a role in activating sperm motility within the SPAP complex. The APOA1 gene is closely associated with other apolipoprotein genes on chromosome 11. mutations in the APOA1 gene can lead to HDL deficiency, Tangier disease, and non-neuropathic amyloidosis. Higher levels of APOA1 have been linked to asthma and atopy.

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  • Sku: PSB-cyt-661-20µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Apolipoprotein A-I, His Tag is a protein belonging to the apolipoprotein family, specifically Apolipoprotein A-I. With a molecular weight of approximately 28 kDa, this protein plays a key role in lipid metabolism and cholesterol transport. The His Tag allows for easy purification and detection of the protein in various research applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
30.3 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The APOA1 solution containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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APOA1, His

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