APEX1

Alternative Names: AP endonuclease 1, APE, APE-1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APEX nuclease (multifunctional DNA repair enzyme) 1, APX, APEN, apurinic/apyrimidinic exonuclease, Apurinic-apyrimidinic endonuclease 1, deoxyribonuclease (apurinic or apyrimidinic), EC 4.2.99.18, HAP1, multifunctional DNA repair enzyme, Protein REF-1, REF1, redox factor 1.

APEX1 is a crucial enzyme involved in base excision repair, responsible for cutting out damaged DNA sites. Additionally, APEX1 enhances the DNA binding ability of various transcription factors related to cancer development. It plays a role in the cellular response to oxidative stress, shielding cells from the harmful effects of oxidizing agents.

  •
  • Sku: PSB-enz-059-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human APEX nuclease-1 is a protein belonging to the apurinic/apyrimidinic endonuclease (APE) family, which plays a key role in DNA repair mechanisms. This enzyme has a molecular weight of approximately 36 kDa and is involved in the removal of abasic sites in DNA, contributing to the maintenance of genomic stability. Recombinant Human APEX nuclease-1 is commonly used in research settings to study DNA repair processes and investigate its potential therapeutic applications in various diseases.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 36.9 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation APEX1 Human solution (1mg/ml) containing 20mM Tris-HCl pH-8, 2mM DTT, 0.2M NaCl, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AAGEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGLASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPLADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSKIRSKALG SDHCPITLYL AL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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APEX1
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