ALAD

Alternative Names: delta-aminolevulinic acid dehydratase, Aminolevulinate delta-dehydratase, Porphobilinogen synthase, ALADH, PBGS, EC 4.2.1.24.

ALAD forms porphobilinogen from delta-aminolevulinate, which is a crucial step in the biosynthesis of heme and other hemoproteins. This enzyme requires zinc for its function and consists of 8 identical subunits. Its activity is hindered by lead, leading to increased susceptibility to lead poisoning and acute hepatic porphyria in individuals with mutations in the ALAD gene.

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  • Sku: PSB-enz-586-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Aminolevulinate Dehydratase is an enzyme that plays a critical role in the biosynthesis of heme, a crucial component of hemoglobin. Belonging to the lyase family, this enzyme has a molecular weight of approximately 39 kDa. Aminolevulinate dehydratase catalyzes the conversion of 5-aminolevulinic acid to porphobilinogen, a key step in the heme biosynthetic pathway. Its recombinant form is commonly used in research and diagnostic applications due to its high specificity and activity.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
38.8 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ALAD solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMQPQSV LHSGYFHPLL RAWQTATTTL NASNLIYPIF VTDVPDDIQP ITSLPGVARY GVKRLEEMLR PLVEEGLRCV LIFGVPSRVP KDERGSAADS EESPAIEAIH LLRKTFPNLL VACDVCLCPY TSHGHCGLLS ENGAFRAEES RQRLAEVALA YAKAGCQVVA PSDMMDGRVE AIKEALMAHG LGNRVSVMSY SAKFASCFYG PFRDAAKSSP AFGDRRCYQL PPGARGLALR AVDRDVREGA DMLMVKPGMP YLDIVREVKD KHPDLPLAVY HVSGEFAMLW HGAQAGAFDL KAAVLEAMTA FRRAGADIII TYYTPQLLQW LKEE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ALAD

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