AKR1C3, His

Alternative Names: DD3, DDX, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, AKR1C3, Aldo-keto reductase family 1 member C3, 3-alpha-HSD type 2, 17-beta-HSD 5, PGFS, DD-3.

AKR1C3 belongs to the aldo/keto reductase superfamily, which consists of over 40 proteins. This enzyme utilizes NADH and/or NADPH as cofactors to convert aldehydes and ketones into their corresponding alcohols. AKR1C3 has a diverse substrate specificity, catalyzing the reduction of prostaglandin D2, PGH2, and phenanthrenequinone, as well as the oxidation of 9alpha,11beta-PGF2 to PGD2. AKR1C3 is implicated in allergic diseases such as asthma, plays a role in controlling cell growth and differentiation, and is involved in adrenal testosterone production. The expression of AKR1C3 is influenced by metabolic disease, with levels decreasing significantly in response to weight loss induced by diet and correlating with leptin levels.

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  • Sku: PSB-enz-406-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human Aldo-Keto Reductase Family 1 Member C3, His Tag is a protein belonging to the aldo-keto reductase family. This protein has a molecular weight of approximately 37 kDa. It is commonly used in research and pharmaceutical applications due to its role in catalyzing the reduction of aldehydes and ketones. The addition of a histidine tag allows for easy purification and detection of the protein.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity: approximately < 0.1 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The AKR1C3 solution contains 20mM Tris-HCl pH-8 and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKPGLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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AKR1C3, His
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