AKR1C1, His

Alternative Names: 20-alpha-HSD, 2-ALPHA-HSD, AKR1C1, Aldo-keto reductase family 1 member C1, C9, Chlordecone reductase homolog HAKRC, DD1, DD1/DD2, DDH, DDH1, Dihydrodiol dehydrogenase 1/2, H-37, HBAB, High-affinity hepatic bile acid-binding protein, Indanol dehydrogenase, MGC8954, MBAB, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase.

AKR1C1 is a member of the aldo/keto reductase superfamily, which includes more than 40 enzymes that catalyze the conversion of aldehydes and ketones to alcohols using NADH and/or NADPH as cofactors. AKR1C1 specifically catalyzes the conversion of progesterone to its inactive state, as well as 20-alpha-hydroxy progesterone. In the liver and intestine, AKR1C1 also plays a role in bile transfer and monitoring intrahepatic bile acid concentrations, although it has low bile-binding ability. Additionally, AKR1C1 is involved in myelin formation.

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  • Sku: PSB-enz-496-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human Aldo-Keto Reductase Family 1 Member C1, His Tag is a protein belonging to the Aldo-Keto Reductase family. It is commonly used in research and biotechnology applications due to its enzymatic properties. This protein has a molecular weight of approximately 36 kDa. Aldo-Keto Reductase Family 1 Member C1, His Tag is a valuable tool for studying various biochemical pathways and processes in the field of molecular biology.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C. Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The AKR1C1 protein solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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AKR1C1, His
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