AKR1B1

Alternative Names: Aldehyde Reductase, EC 1.1.1.21, ALR2, ALDR1, MGC1804, Aldo-keto reductase family1 member B1, Aldose Reductase, AKR1B1, AR, ADR.

AKR1B1, a member of the aldo/keto reductase superfamily, is involved in catalyzing the reduction of various aldehydes, including the aldehyde form of glucose. This enzyme plays a key role in diabetic complications by converting glucose to sorbitol. Additionally, AKR1B1 is responsible for reducing carbonyl-containing compounds to their corresponding alcohols in an NADPH-dependent manner. Studies using transgenic mice have shown that AKR1B1 is implicated in ischemic injury and the impairment of functional and metabolic recovery post ischemia. Furthermore, AKR1B1 serves as a crucial regulator of TNF-alpha-induced apoptotic signaling in endothelial cells by mediating an increase in the expression of adhesion molecules and promoting binding of monocytes to the endothelium.

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  • Sku: PSB-enz-390-10µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human Aldose Reductase is an enzyme belonging to the aldo-keto reductase family. It plays a role in the reduction of glucose to sorbitol in the polyol pathway. With a molecular weight of approximately 36 kDa, this enzyme is involved in the metabolism of sugars and aldehydes, making it a crucial player in various biochemical processes. Its recombinant form is commonly used in research and drug development for its potential therapeutic applications in diabetic complications and other diseases related to sugar metabolism.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37°C. Specific activity is > 800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The 1mg/ml protein solution contains 20mM Tris-HCl buffer pH 8, 10% glycerol, and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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AKR1B1
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