AICDA

Alternative Names: Activation-induced cytidine deaminase, AICDA, AID, ARP2, Cytidine aminohydrolase, CDA2, HIGM2, Single-stranded DNA cytosine deaminase.

Activation-Induced Cytidine Deaminase (AICDA) is a member of the cytidine deaminase family and plays a key role in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. This enzyme is essential for various steps in B-cell terminal differentiation that are required for effective antibody responses. Additionally, AICDA is involved in the epigenetic regulation of gene expression through DNA demethylation. It is primarily expressed in lymph nodes and tonsils.

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  • Sku: PSB-enz-651-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Activation-Induced Cytidine Deaminase (AID) is an enzyme belonging to the cytidine deaminase family. With a molecular weight of approximately 24 kDa, AID plays a significant role in antibody diversification by catalyzing the deamination of cytidine to uridine in DNA. This process is crucial for somatic hypermutation and class switch recombination in B cells, ultimately contributing to the generation of a diverse antibody repertoire. AID is a key player in the adaptive immune response and is essential for the development of effective immune defenses against pathogens.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
26.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The AICDA solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENHERTFKAWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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AICDA

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