Ag85A

Alternative Names: Mycolyl transferase 85A, Ronectin-binding protein A.

Antigen 85A is part of the antigen 85 complex, which includes Antigen 85B and Antigen 85C. The enzymes in the antigen 85 complex have mycolyltransferase activity and are responsible for producing the cord factor (trehalose 6,6'-dimycolate, TDM), a glycolipid found in the mycobacterial cell wall. The cord factor is essential for maintaining the integrity of the mycobacterial cell wall and plays a role in the pathogenesis of the bacillus. Antigen 85A specifically synthesizes TDM from two molecules of trehalose-6'-monomycolate (TMM).

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  • Sku: PSB-pro-1076-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

The Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A is a prominent component of the cell wall in Mycobacterium tuberculosis, the bacterium responsible for causing tuberculosis. Belonging to the Antigen 85 family, this protein plays a crucial role in the pathogenesis and immunogenicity of the disease. With a molecular weight of approximately X kDa, it is involved in the biosynthesis and transport of mycolic acids, which are essential for the integrity and virulence of the bacterium. Its significance lies in its potential as a diagnostic marker and vaccine candidate for tuberculosis.

Current Lead Time

7-14 Days

Product Specifications

Species
Mycobacterium
Expression System Baculovirus
Purity 95 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
32.8 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The Ag85A solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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Ag85A

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