ADI1

Alternative Names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase, APL1, ARD, Fe-ARD, FLJ10913, HMFT1638, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, MTCBP-1, SIPL, Submergence-induced protein-like factor.

Acireductone dioxygenase 1 (ADI1) is a metal-binding enzyme within the acireductone dioxygenase family. It plays a role in methionine salvage, as well as regulating mRNA processing in the nucleus. ADI1 has been found to have different functions based on its localization, with related pseudogenes on chromosomes 8 and 20. ADI1 has also been shown to down-regulate cell migration mediated by MMP14.

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  • Sku: PSB-enz-700-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Acireductone Dioxygenase 1 is a member of the metalloenzyme family that plays a role in the methionine salvage pathway. This enzyme is responsible for the conversion of acireductone to formate and 2-keto-4-methylthiobutyrate. Recombinant Human Acireductone Dioxygenase 1 has a molecular weight of approximately 20 kDa. Its function is crucial in maintaining sulfur amino acid metabolism and cellular redox balance.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
25.6 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ADI1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ADI1

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