ACP5 His

Alternative Names: Tartrate-Resistant Acid Phosphatase, Tartrate-Resistant Acid Phosphatase Type 5, Type 5 Acid Phosphatase, Acid Phosphatase 5, TRAP, TR-AP, ACP5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, TrATPase, EC 3.1.3.2, SPENCDI

Recombinant Human Acid Phosphatase-5 His Tag is a member of the acid phosphatase family, responsible for catalyzing the hydrolysis of phosphate esters in acidic conditions. With a molecular weight of approximately 50 kDa, this enzyme plays a significant role in various cellular processes. The addition of a 5 His Tag allows for easy purification and detection of the protein. This recombinant enzyme is commonly used in research and biotechnological applications for its enzymatic activity and specificity.

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  • Sku: PSB-enz-913-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Acid Phosphatase-5 His Tag is a member of the acid phosphatase family, responsible for catalyzing the hydrolysis of phosphate esters in acidic conditions. With a molecular weight of approximately 50 kDa, this enzyme plays a significant role in various cellular processes. The addition of a 5 His Tag allows for easy purification and detection of the protein. This recombinant enzyme is commonly used in research and biotechnological applications for its enzymatic activity and specificity.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 95% by SDS-PAGE
Activity
Specific activity is > 5,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
35.1 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ACP5 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARPHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ACP5 His

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