ACKA

Alternative Names: Acetokinase, Acetate kinase, ack, ackA, ACKA, Acetate kinase A, propionate kinase 2

Recombinant E.Coli Acetate Kinase is an enzyme that belongs to the acetate and butanoate metabolism family. This protein plays a key role in the phosphorylation of acetate to form acetyl phosphate, an essential step in the metabolic pathways of many organisms. With a molecular weight of approximately 45 kDa, Recombinant E.Coli Acetate Kinase is a crucial component in the regulation of carbon metabolism in bacteria. Its enzymatic activity is vital for the efficient utilization of acetate as a carbon source in E.Coli and other microorganisms.

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  • Sku: PSB-pka-063-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant E.Coli Acetate Kinase is an enzyme that belongs to the acetate and butanoate metabolism family. This protein plays a key role in the phosphorylation of acetate to form acetyl phosphate, an essential step in the metabolic pathways of many organisms. With a molecular weight of approximately 45 kDa, Recombinant E.Coli Acetate Kinase is a crucial component in the regulation of carbon metabolism in bacteria. Its enzymatic activity is vital for the efficient utilization of acetate as a carbon source in E.Coli and other microorganisms.

Current Lead Time

7-14 Days

Product Specifications

Species
E.Coli
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ACKA protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ACKA

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