ACHE

Alternative Names: Acetylcholinesterase, AChE, ACEE, ACES_HUMAN, ACHE, ARACHE, N-ACHE, VT, Acetylcholinesterase isoform E4-E6

Acetylcholinesterase (ACHE) is a type-B carboxylesterase/lipase that breaks down acetylcholine and other choline esters involved in neurotransmission. It is found on the post-synaptic membrane where it terminates nerve signal transmission by hydrolyzing acetylcholine.

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  • Sku: PSB-enz-1174-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant human acetylcholinesterase is a protein belonging to the cholinesterase enzyme family. It plays a vital role in the breakdown of the neurotransmitter acetylcholine, regulating nerve signal transmission in the central and peripheral nervous systems. With a molecular weight of approximately 60-70 kDa, this enzyme is crucial for maintaining proper neuromuscular function and cognitive processes. Its recombinant form is commonly used in research and therapeutic applications for studying neurological disorders and developing treatments targeting cholinergic pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System HEK 293
Purity 95.0 by SDS-PAGE
Activity
Specific activity is > 6,000 nmol/min/ug. Defined by the amount of enzyme that cleaves  1 nmole of acetylthiocholine per minute at pH 7.5 at 25˚C.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ACHE solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

DGSEGREDAE LLVTVRGGRL RGIRLKTPGG PVSAFLGIPF AEPPMGPRRF LPPEPKQPWS GVVDATTFQS VCYQYVDTLY PGFEGTEMWN PNRELSEDCL YLNVWTPYPR PTSPTPVLVW IYGGGFYSGA SSLDVYDGRF LVQAERTVLV SMNYRVGAFG FLALPGSREA PGNVGLLDQR LALQWVQENV AAFGGDPTSV TLFGESAGAA SVGMHLLSPP SRGLFHRAVL QSGAPNGPWA TVGMGEARRR ATQLAHLVGC PPGGTGGNDT ELVACLRTRP AQVLVNHEWH VLPQESVFRF SFVPVVDGDF LSDTPEALIN AGDFHGLQVL VGVVKDEGSY FLVYGAPGFS KDNESLISRA EFLAGVRVGV PQVSDLAAEA VVLHYTDWLH PEDPARLREA LSDVVGDHNV VCPVAQLAGR LAAQGARVYA YVFEHRASTL SWPLWMGVPH GYEIEFIFGI PLDPSRNYTA EEKIFAQRLM RYWANFARTG DPNEPRDPKA PQWPPYTAGA QQYVSLDLRP LEVRRGLRAQ ACAFWNRFLP KLLSATDTLD EAERQWKAEF HRWSSYMVHW KNQFDHYSKQ DRCSDLHHHH HH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ACHE

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