ACE2 (18-740)

Alternative Names: Not Available

ACE-2 is an enzyme found in various organs that acts as a receptor for SARS coronaviruses, including SARS-CoV-2. The coronavirus spike glycoprotein plays a critical role in viral infection by facilitating fusion of viral and cellular membranes. ACE2 has two main domains, with the C-domain being important for attachment of SARS-CoV and MERS-CoV to their receptors. ACE2 is a transmembrane antigen with a catalytic site that degrades Ang I and Ang II to produce smaller peptides.

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  • Sku: PSB-enz-1125-50µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Angiotensin Converting Enzyme 2 (18-740 a.a.) is a protein belonging to the angiotensin-converting enzyme family. With a molecular weight of approximately 80 kDa, this enzyme plays a key role in the renin-angiotensin system by cleaving angiotensin II into angiotensin 1-7, thereby regulating blood pressure and fluid balance. Its therapeutic potential in treating cardiovascular and respiratory diseases has garnered significant interest in recent research.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System HEK 293
Purity 90 by SDS-PAGE
Activity
Determined by its binding ability in a functional ELISA using SARS-CoV-2 Spike RBD. The ED50 range ≤ 100ng/ml. Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH / min. at pH-7.5, at 25C
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Oligomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ACE2 Human protein 0.5mg/ml solution is supplied in PBS, pH7.4, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

DGS QSTIEEQ AKTFLDKFNH EAEDLFYQSS LASWNYNTNI TEENVQNMNN AGDKWSAFLK EQSTLAQMYP LQEIQNLTVK LQLQALQQNG SSVLSEDKSK RLNTILNTMS TIYSTGKVCN PDNPQECLLL EPGLNEIMAN SLDYNERLWA WESWRSEVGK QLRPLYEEYV VLKNEMARAN HYEDYGDYWR GDYEVNGVDG YDYSRGQLIE DVEHTFEEIK PLYEHLHAYV RAKLMNAYPS YISPIGCLPA HLLGDMWGRF WTNLYSLTVP FGQKPNIDVT DAMVDQAWDA QRIFKEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQPFLLRN GANEGFHEAV GEIMSLSAAT PKHLKSIGLL SPDFQEDNET EINFLLKQAL TIVGTLPFTY MLEKWRWMVF KGEIPKDQWM KKWWEMKREI VGVVEPVPHD ETYCDPASLF HVSNDYSFIR YYTRTLYQFQ FQEALCQAAK HEGPLHKCDI SNSTEAGQKL FNMLRLGKSE PWTLALENVV GAKNMNVRPL LNYFEPLFTW LKDQNKNSFV GWSTDWSPYA DQSIKVRISL KSALGDKAYE WNDNEMYLFR SSVAYAMRQY FLKVKNQMIL FGEEDVRVAN LKPRISFNFF VTAPKNVSDI IPRTEVEKAI RMSRSRINDA FRLNDNSLEF LGIQPTLGPP NQPPVSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSHEDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQVYTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVMHEALHNHYTQ KSLSLSPGKH HHHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ACE2 (18-740)

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