ACADM

Alternative Names: ACADM, ACAD1, CAD, MCAD, MCADH, EC=1.3.99.3, Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, FLJ18227, FLJ93013, FLJ99884.

ACADM enzyme is crucial for breaking down medium-chain fatty acids into energy, particularly during fasting. It is primarily located in the mitochondria of various tissues, with a focus on the liver.

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  • Sku: PSB-enz-529-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Acyl-Coenzyme A Dehydrogenase, C-4 to C-12 is an enzyme belonging to the acyl-CoA dehydrogenase family, responsible for catalyzing the oxidation of fatty acyl-CoA esters in the mitochondria. With a molecular weight of approximately 45 kDa, this enzyme plays a crucial role in the beta-oxidation pathway by facilitating the breakdown of fatty acids into acetyl-CoA, which can then be utilized for energy production. Its specificity for fatty acyl-CoA substrates ranging from 4 to 12 carbons in length makes it a key player in lipid metabolism.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ACADM (0.5mg/ml) protein solution containing 20mM Tris-HCl pH-7.5, 0.1M NaCl & 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKANRQREPG LGFSFEFTEQ QKEFQATARK FAREEIIPVA AEYDKTGEYP VPLIRRAWEL GLMNTHIPEN CGGLGLGTFD ACLISEELAY GCTGVQTAIE GNSLGQMPII IAGNDQQKKK YLGRMTEEPL MCAYCVTEPG AGSDVAGIKT KAEKKGDEYI INGQKMWITN GGKANWYFLL ARSDPDPKAP ANKAFTGFIV EADTPGIQIG RKELNMGQRC SDTRGIVFED VKVPKENVLI GDGAGFKVAM GAFDKTRPVV AAGAVGLAQR ALDEATKYAL ERKTFGKLLV EHQAISFMLA EMAMKVELAR MSYQRAAWEV DSGRRNTYYA SIAKAFAGDI ANQLATDAVQ ILGGNGFNTE YPVEKLMRDA KIYQIYEGTS QIQRLIVARE HIDKYKN.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ACADM

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